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KMID : 0613820070170010045
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Journal of Life Science
2007 Volume.17 No. 1 p.45 ~ p.50
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Biological Activity of Tumor Necrosis Factor-α Secreted from Smooth Muscle Cell Overexpressing FADD
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Kim Sun-Mi
Lee Kyeong-Ah
Kim Koan-hoi
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Abstract
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This study investigated biological activity of tumor necrosis factor (TNF)-α secreted from smooth muscle cell (SMC) destined for death by expressing Fas associated death domain containing protein (FADD) (FADD-SMC) when the cells are grown without tetracycline in culture medium. In the absence of tetracycline the FADD-SMC secreted approximately 1000 pg/㎖ TNF-α, whereas hardly detectable amount of the cytokine existed in the presence of tetracycline. The culture medium collected from the FADD-SMC grown in the absence of tetracycline increased phosphorylated form of p38 MAPK and up-regulated nuclear factor kappa B (NF-κB). The medium collected without tetracycline also caused death of L929 cells. Depletion of TNF-α with the soluble TNF receptor (sTNFR) inhibited the phosphorylation of p38 MAPK, the up-regulation of NF-κB activity and the death activity of the medium collected from FADD-SMC in the absence of tetracycline. These results indicate that TNF-α secreted from SMC undergoing death is biologically active and can affect cellular function.
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KEYWORD
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Apoptosis, p38 MAPK, Smooth muscle cell, Tumor necrosis factor-α
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